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dc.contributor.authorLoubnan, Georgette
dc.date.accessioned2007-08-10T13:43:35Z
dc.date.available2007-08-10T13:43:35Z
dc.date.issued2007-08-10T13:43:35Z
dc.identifier.urihttps://hdl.handle.net/1813/8168
dc.description.abstractThe differentiation between gram negative and gram positive bacteria was investigated based on the identification of specific sequences in the 16S rRNA of pathogenic bacteria. The rRNA sequences were aligned and detection probes were identified using the AlleleID software. Subsequently, the specificity of the probes was checked using the basic local alignment sequence tool of the NCBI data bank. One 23 nt long probe sequence was identified that is able to bind to 79% of the selected gram negative bacteria where as the gram positive probe can only detect 64% of the selected gram positive bacteria. Unfortunately, it was impossible to identify a second probe that was specific and common to all gram negative and even less for all gram positive bacteria. Further experiment will be conducted to test the sensitivity of the probes and their limits of detection.en_US
dc.description.sponsorshipBaeumner's LAben_US
dc.format.extent852484 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.subjectIdentifiation of bacterial gram typeen_US
dc.subjectrRNA detectionen_US
dc.titleDifferentiation of bacterial gram type via rRNA detectionen_US
dc.typedissertation or thesisen_US


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