MRE11 is Unlikely to be an Essential Component of the Saccharomyces cerevisiae Mismatch Repair Pathway
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hMRE11 has recently been implicated as a primary factor in human mismatch repair (MMR), a process which acts to improve DNA replication fidelity. Mutations in hMRE11 have been identified in human nonpolyposis colorectal cancer (HNPCC) related tumors and in vivo mutator assays suggest that knockdown of this gene causes frameshift mutations. I report that the deletion of MRE11 from the Saccharomyces cerevisiae genome increases the mutation rate but does not significantly enhance the rate of frameshift mutations as seen in other yeast MMR mutants. In addition, unlike in mammalian systems, I was unable to observe yeast two-hybrid interaction between MRE11 and MLH1. This combined evidence suggests that MRE11 is unlikely to be a major player in yeast MMR.
dissertation or thesis