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dc.contributor.authorYang, Wenhao
dc.date.accessioned2019-10-15T16:50:06Z
dc.date.available2021-08-29T06:00:23Z
dc.date.issued2019-08-30
dc.identifier.otherYang_cornell_0058O_10722
dc.identifier.otherhttp://dissertations.umi.com/cornell:10722
dc.identifier.otherbibid: 11050679
dc.identifier.urihttps://hdl.handle.net/1813/67694
dc.description.abstractLyme disease, also known as Lyme Borreliosis, is caused by bacteria of the Borrelia type, and it is the most common vector-borne infectious disease spread by ticks in the densely populated Northern Hemisphere including Asia, Europe, and North America. Lyme disease can lead to significant pains and sufferings, making it one of the most insufferable diseases. If left untreated, infected patients can develop symptoms as serious as paralysis, chronic shooting pain, heart problems among others. Early detection is essential in proper Lyme disease treatment. The current technique used to detect Lyme disease is a two-step approach including an ELISA (enzyme-linked immunosorbent assay) test and a confirmatory Western blot test. However, ELISA is not an ideal test because it relies on the use of secondary antibodies to detect primary antibodies. Due to non-specific binding of secondary antibodies, ELISA suffers from a high false-positive rate, making a positive result unreliable and must be confirmed by an additional Western blot test. In addition, since secondary antibodies are specific to species, a different Lyme test protocol is required for each different species. Lastly, because secondary antibodies are not cheap and not always readily available, an ELISA-based Lyme test is unnecessarily expensive and slow. There is, however, a novel technique that overcomes the disadvantages of the ELISA-based Lyme test. By using the antibody-catalyzed water oxidation pathway (ACWOP), a novel biosensor can be designed to directly detect the presence of primary antibodies to Borrelia without the use of enzyme-tagged secondary antibodies. In ACWOP, the intrinsic catalytic power of all antibodies regardless of species is utilized to oxidize water into hydrogen peroxide for detection. Addition of readout reagents in the presence of hydrogen peroxide can then generate either colorimetric or fluorometric signals that can be easily detected. Since ACWOP eliminates the use of species-specific secondary antibodies, the novel Lyme test based on ACWOP will have higher specificity, more convenience and more affordability, and most importantly, unify human and veterinary serological testing.
dc.language.isoen_US
dc.subjectLyme Disease
dc.subjectSerological Test
dc.subjectChemical engineering
dc.subjectMicrofluidic device
dc.subjectMaterials Science
dc.subjectAntibody
dc.subjectMechanical engineering
dc.subjectACWOP
dc.subjectbiosensor
dc.titleDeveloping a Novel Species-Independent Biosensor for Lyme Disease via the Antibody-Catalyzed Water Oxidation Pathway
dc.typedissertation or thesis
thesis.degree.disciplineChemical Engineering
thesis.degree.grantorCornell University
thesis.degree.levelMaster of Science
thesis.degree.nameM.S., Chemical Engineering
dc.contributor.chairKirby, Brian
dc.contributor.committeeMemberDaniel, Susan
dcterms.licensehttps://hdl.handle.net/1813/59810
dc.identifier.doihttps://doi.org/10.7298/r3jy-py15


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