Microsatellites for Oenothera gayleana and O. hartwegii subsp. filifolia (Onagraceae), and their utility in section Calylophus
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Lewis, E. M.; Fant, J. B.; Moore, M. J.; Hastings, Amy P.; Larson, E. L.; Agrawal, Anurag A.; Skogen, K. A.
Premise of the study: Eleven nuclear and four plastid microsatellite markers were screened for two gypsum endemic species, Oenothera gayleana and O. hartwegii subsp. filifolia, and tested for cross?amplification in the remaining 11 taxa within Oenothera sect. Calylophus (Onagraceae). Methods and Results: Microsatellite markers were tested in two to three populations spanning the ranges of both O. gayleana and O. hartwegii subsp. filifolia. The nuclear microsatellite loci consisted of both di? and trinucleotide repeats with one to 17 alleles per population. Several loci showed significant deviation from Hardy–Weinberg equilibrium, which may be evidence of chromosomal rings. The plastid microsatellite markers identified one to seven haplotypes per population. The transferability of these markers was confirmed in all 11 taxa within Oenothera sect. Calylophus. Conclusions: The microsatellite loci characterized here are the first developed and tested in Oenothera sect. Calylophus. These markers will be used to assess whether pollinator foraging distance influences population genetic parameters in predictable ways.
Applications in Plant Sciences
Funding was provided by the National Science Foundation (DEB 1342873 to K.A.S. and J.B.F., DEB 1054539 to M.J.M., and DEB-1513839 to A.A.A.), the National Geographic Society (M.J.M.), Oberlin College, a Botanical Society of America Graduate Student Research Award (E.M.L.), and the Chicago Botanic Garden.
Botanical Society of America
gypsum endemism; microsatellites; Oenothera sect. Calylophus; Onagraceae; population genetics
Lewis, E. M., Fant, J. B., Moore, M. J., Hastings, A. P., Larson, E. L., Agrawal, A. A., & Skogen, K. A. (2016). Microsatellites for Oenothera gayleana and O. hartwegii subsp. filifolia (Onagraceae), and their utility in section Calylophus. Applications in Plant Sciences, 4(2), 1500107.