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An investigation of the role of enzyme phosphopantetheinyl hydrolase in mycobacteria

Author
Geiger, Annie
Abstract
Phosphopantetheinyl transferase (PptT) is an essential enzyme in Mycobacterium tuberculosis (Mtb) that adds a pantothenate group onto carrier proteins using coenzyme A (CoA), changing them from the apo to holo enzyme configuration. Encoded for in the same operon, phosphopantetheinyl hydrolase (PptH) is a newly identified enzyme that counterbalances this reaction by removing the pantetheine group from the holo-carrier protein, resulting in an apo-carrier protein. Because PptH appears to operate a futile cycle in the cell, its purpose remains unknown. In this project, I aimed to understand the functions of PptH and the conditions in which it becomes essential in the mycobacterial cell through a series of chemical stress tests and an analysis of binding partners of PptH. Select chemical stress growth assays indicated that Mycobacterium smegmatis (Msm) strains encoding putative PptH loss-of-function (LOF) mutations possess impaired cell wall integrity. CRISPRi was used to test for synthetic lethality in the proposed role of PptH in a CoA salvage pathway. Co-IPs were not able to show that PptH and its neighboring enzyme, PptT are binding partners. These results gleam insight into mycobacterial biology, the functions of PptH, and its efficacy as a putative drug target for tuberculosis.
Date Issued
2019-05Subject
Biological sciences honors program; Mycobacteria; tuberculosis; Cocnzyme A metabolism; phosphopantetheinyl transferase; phosphopantetheinyl hydrolase.; mycobucterial cell wall; CRISPRi; enzyme function; novel mechanism of antimicrobial resistance
Degree Discipline
Biological Sciences
Degree Name
B.S., Biological Sciences
Degree Level
Bachelor of Science
Type
dissertation or thesis