Show simple item record

dc.contributor.authorPanda, Oishika
dc.date.accessioned2018-10-23T13:22:23Z
dc.date.available2020-06-04T06:00:53Z
dc.date.issued2018-05-30
dc.identifier.otherPanda_cornellgrad_0058F_10710
dc.identifier.otherhttp://dissertations.umi.com/cornellgrad:10710
dc.identifier.otherbibid: 10489469
dc.identifier.urihttps://hdl.handle.net/1813/59384
dc.description.abstractChemical communication forms an integral part of any organism’s overall health, longevity, social interactions and various other aspects of its biology. Chemical cues produced by different organisms are products of primary and secondary metabolism, and are highly specific in their structural assembly and signaling functions. Minute differences in structures and functions of these metabolites can, for example, help an organism distinguish between pathogenic and beneficial microbes, cause the organism to extend or reduce its lifespan, signal availability or lack of sustenance, and provide snapshots of its metabolic state. As such, it is crucial to annotate structures and functions of the underlying chemical causes behind biological effects, understand how subtle structural differences cause dramatic changes in observed phenotypes, and elucidate their biosynthetic mechanisms to learn how biological information is encoded in these signaling molecules. Ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) is rapidly becoming an indispensable tool in the study of signaling molecules using comparative metabolomics. This technique far outdoes classical activity-guided fractionation methods to identify chemical cues in biological systems in terms of required time, ease of use and sensitivity. The use of both activity-guided fractionation and UHPLC-MS/MS techniques to elucidate structures of signaling molecules involved in host-pathogen interactions between nematodes and bacteria, and mitochondrion-mediated longevity in nematode are discussed in this dissertation. Forward genetic approaches to identify biosynthetic enzymes responsible for the highly specific assembly of nematode-derived modular metabolites, and validation of their biosynthetic functions are also described. Finally, an untargeted metabolomics approach combining high resolution UHPLC-MS/MS data with genome-wide association studies to annotate the nematode metabolome and identify candidate biosynthetic genes for various metabolites is evaluated.
dc.language.isoen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectCaenorhabditis elegans
dc.subjectPristionchus pacificus
dc.subjectmetabolomics
dc.subjectGWAS
dc.subjectChemistry
dc.subjectMitochondria
dc.subjectbiosynthesis
dc.titleIDENTIFICATION AND CHARACTERIZATION OF SIGNALING MOLECULES IN NEMATODES AND BACTERIA USING LC-MS-BASED COMPARATIVE METABOLOMICS
dc.typedissertation or thesis
thesis.degree.disciplineChemistry and Chemical Biology
thesis.degree.grantorCornell University
thesis.degree.levelDoctor of Philosophy
thesis.degree.namePh. D., Chemistry and Chemical Biology
dc.contributor.chairSchroeder, Frank
dc.contributor.committeeMemberLin, Hening
dc.contributor.committeeMemberLee, Siu Sylvia
dcterms.licensehttps://hdl.handle.net/1813/59810
dc.identifier.doihttps://doi.org/10.7298/X41C1V3F


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International

Statistics