Show simple item record

dc.contributor.authorLiu, Yichang
dc.date.accessioned2018-10-23T13:21:54Z
dc.date.available2020-06-04T06:00:42Z
dc.date.issued2018-05-30
dc.identifier.otherLiu_cornellgrad_0058F_10780
dc.identifier.otherhttp://dissertations.umi.com/cornellgrad:10780
dc.identifier.otherbibid: 10489424
dc.identifier.urihttps://hdl.handle.net/1813/59339
dc.description.abstractListeria monocytogenes presents a challenge to food safety and public health worldwide. This foodborne pathogen is known to cause listeriosis, a serious invasive disease that particularly affects susceptible populations including immunocompromised individuals, newborns, pregnant women, and elderly people. New approaches for prevention and treatment of listeriosis require better understanding of mechanisms that enable L. monocytogenes to survive and thrive under a wide range of environmental conditions. One key regulatory mechanism of this Gram-positive bacterium involves the differential expression of different sets of genes by alternative sigma factors. In these studies, we employed next-generation sequencing tools to explore the regulatory network controlled by two alternative sigma factors, σB and σH. Specifically, our studies focused on (i) the regulatory roles of σB and σH, in the absence of other alternative sigma factors, (ii) the contribution of σB regulon members in important biological processes, and (iii) the development of a new transposon sequencing tool in L. monocytogenes. Our RNA sequencing data indicate that σH regulates a number of competence genes; We also found that in addition to stress response and virulence, σB has a broad role of resilience. For instance, σB regulates the metabolism of macronutrients and micronutrients to succeed in the intracellular environment where many nutrients are limited. Lastly, we designed a vector for a new transposon delivery system in L. monocytogenes. Overall, these findings provide a more complete picture of the regulatory network in L. monocytogenes.
dc.language.isoen_US
dc.subjectFood science
dc.subjectRNA-Seq
dc.subjectListeria monocytogenes
dc.subjectregulon
dc.subjectsigB
dc.subjectsigH
dc.titleUSE OF NEXT-GENERATION SEQUENCING TOOLS TO EXPLORE THE REGULATORY NETWORK OF LISTERIA MONOCYTOGENES
dc.typedissertation or thesis
thesis.degree.disciplineFood Science and Technology
thesis.degree.grantorCornell University
thesis.degree.levelDoctor of Philosophy
thesis.degree.namePh. D., Food Science and Technology
dc.contributor.chairWiedmann, Martin
dc.contributor.committeeMemberGrohn, Yrjo Tapio
dc.contributor.committeeMemberTurvey, Calum G.
dcterms.licensehttps://hdl.handle.net/1813/59810
dc.identifier.doihttps://doi.org/10.7298/X45D8Q2K


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Statistics