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dc.contributor.authorTruhlar, Allison Mary
dc.date.accessioned2018-04-26T14:17:50Z
dc.date.available2019-09-11T06:01:55Z
dc.date.issued2017-08-30
dc.identifier.otherTruhlar_cornellgrad_0058F_10325
dc.identifier.otherhttp://dissertations.umi.com/cornellgrad:10325
dc.identifier.otherbibid: 10361632
dc.identifier.urihttps://hdl.handle.net/1813/56955
dc.description.abstractThere is great interest in identifying manure management techniques to minimize the persistence of pathogenic Escherichia coli in agricultural fields and, thereby, decrease the risk of downstream contamination and human infection. The first two chapters of this dissertation seek to determine the how the agricultural environmental variable of manure application method shapes the genetic and phenotypic population structure of E. coli. First, we conducted a field and laboratory experiment that demonstrated the expression of extracellular fibers called curli, which are linked to environmental persistence of E. coli, was linked to surface-application of manure, as opposed to incorporation into the soil. Second, we applied whole genome sequencing technology to isolates collected from laboratory microcosms with differing manure application treatments. We found no systematic genomic differences (i.e. individual-level selection) that could be explained by week or manure application treatment. As higher education institutions offer online courses to growing audiences, there is increasing desire to understand how best to engage students. The third chapter of this dissertation examines the effects of assigning chat roles and facilitating self and group reflection on student-content and student-student interaction outcomes in synchronous online chats. Group reflections were the only intervention that had a significant effect on both outcomes.
dc.language.isoen_US
dc.subjectOnline discussions
dc.subjectWhole genome sequencing
dc.subjectEducational technology
dc.subjectMicrobiology
dc.subjectCurli
dc.subjectEscherichia coli
dc.subjectGroup reflection
dc.subjectManure application
dc.titleSURVIVAL OF ESCHERICHIA COLI IN AGRICULTURAL SOILS AND STUDENT ENGAGEMENT IN ONLINE DISCUSSIONS
dc.typedissertation or thesis
thesis.degree.disciplineBiological and Environmental Engineering
thesis.degree.grantorCornell University
thesis.degree.levelDoctor of Philosophy
thesis.degree.namePh. D., Biological and Environmental Engineering
dc.contributor.chairWalter, Michael Todd
dc.contributor.committeeMemberWilliams, Kimberly
dc.contributor.committeeMemberHay, Anthony G.
dc.contributor.committeeMemberRichardson, Ruth E.
dcterms.licensehttps://hdl.handle.net/1813/59810
dc.identifier.doihttps://doi.org/10.7298/X4WH2N5C


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