Show simple item record

dc.contributor.advisorNixon, Alan
dc.contributor.authorBallantyne, Rachel
dc.date.accessioned2017-06-21T14:24:30Z
dc.date.available2017-06-21T14:24:30Z
dc.date.issued2013-05
dc.identifier.urihttps://hdl.handle.net/1813/51502
dc.description.abstractMesenchymal stem cells (MSCs) have exciting medical potential, especially those that are genetically modified. Non-viral methods for genetic modification present several advantages over viral methods, and hence, such methods have been optimized for MSCs from a variety of species. Although equine MSCs have great importance, prior to this study, effective non-viral transfection parameters for these cells had not been determined. Here both chemical and physical transfection methods were optimized for equine MSCs, using red fluorescent protein as a reporter gene. Chemical reagents were optimized for reagent-to-DNA ratio, transfection solution plating volume, and cell density. Additionally, ideal voltage, cell concentration, DNA concentration, buffer, and pulse number settings were discovered for electroporation. The method resulting in highest gene expression was electroporation with one 30-msec pulse at 170 volts in Opti-Mem buffer. This technique resulted in transfection of 54% of the cell population, a percentage that is on the higher end of nonviral MSC transfection efficiencies. The transfection parameters determined in this study will undoubtedly be useful to all researchers who wish to genetically modify equine MSCs.
dc.language.isoen_US
dc.subjectBiological sciences honors program
dc.subjectelectroporation
dc.subjectequine
dc.subjectgene therapy
dc.subjectmesenchymal stem cells
dc.subjectnonviral gene transfer
dc.subjectoptimization
dc.subjecttransfection
dc.subjecttransduction
dc.subjectgenetic modification
dc.subjectlipofection
dc.titleOptimization of non-viral transfection methods for equine mesenchymal stem cells
dc.typedissertation or thesis
thesis.degree.disciplineBiological Sciences
thesis.degree.levelBachelor of Arts
thesis.degree.nameB.A. of Biological Sciences


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Statistics