Exploiting Serum Interactions with Cationic Biomaterials Enables Label-Free Circulating Tumor Cell Isolation
Castellanos, Carlos A
Herein we investigate the role charged biomaterials and fluid dielectric properties have on microfluidic capture and isolation of circulating tumor cells. We determine that heparan sulfate proteoglycans on cancer cell surfaces are responsible for elevated electric charge of cancer cells compared with white blood cells and that these proteoglycans help mediate adhesive interactions between cells and charged surfaces in albumin-containing fluids. Cancer cell firm adhesion to charged surfaces persists when cells are bathed in up to 1% (w/v) human albumin solution, while white blood cell adhesion is nearly abrogated. As many protocols rely on electrical interactions between cells and biomaterials, our study could reveal a new determinant of efficient adhesion and targeting of specific tissue types in the context of a biological fluid environment.
charged biomaterials; dielectric; serum albumin; Biomedical engineering; Physics; Materials Science; circulating tumor cell
King, Michael R.
MS of Biomedical Engineering
Master of Science
dissertation or thesis