Young (8-week-old) female Syrian hamsters were treated with 4-vinylcyclohexene diepoxide (VCD), which destroys ovarian follicles and functionally accelerates ovarian follicle depletion without compromising general health. VCD-treated and control females were allowed to choose between a dominant and a subordinate male in a Y-maze on the day of proestrus. This collection includes digital video recordings of both VCD-treated and control females in the Y maze. A separate file indicates the treatment status of each animal (male and female) in the videos and includes data on each female's behavior in the Y maze, collected by a blind observer.