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dc.contributor.authorInnocent, Cassandravictoriaen_US
dc.date.accessioned2015-04-06T20:13:39Z
dc.date.available2020-01-27T07:00:37Z
dc.date.issued2015-01-26en_US
dc.identifier.otherbibid: 9154391
dc.identifier.urihttps://hdl.handle.net/1813/39310
dc.description.abstractBursicon is a neurohormone packaged in and secreted by type III synaptic contacts. In comparison to the depth of information describing type I and type II neuromuscular junctions (NMJs), type III NMJs are notably ill-defined. As such, this lab has provided evidence identifying bursicon as a reliable marker in an aim to characterize type III NMJs. Firstly, a comprehensive registry of proteins defining type III NMJs can be compiled through antibody-labeled colocalizations using bursicon as the type III marker. Candidates to be stained with bursicon include established presynaptic markers such as the SNARE proteins. Positional overlap in these dual antibody-staining profiles will be confirmed by sequential-excitation confocal microscopy. This proteomic approach establishes an immunohistological staining profile characterizing type III NMJs. Secondly, this effort in effect also produces a directory identifying proteins involved in vesicle delivery in type III NMJs and can be used to reveal the machinery by which neuropeptides are trafficked and secreted. While what is known about the mechanisms of vesicle trafficking employed by neurotransmitter-releasing type I synapses continues to be expanded, information on these same properties in peptidergic type III NMJs idles. Therefore, using a GFP- v variant fusion protein able to mimic bursicon's packaging and transport in vesicles, this lab has perturbed in vivo delivery of a fluorescent neuropeptide to describe type III synaptic release. In doing so we aim to elucidate the dynamic process specifically employed by type III NMJs when trafficking secretory vesicles through boutons in parallel and add to the breadth of knowledge gathered about the mechanism used during peptide release. vien_US
dc.language.isoen_USen_US
dc.subjectboutonen_US
dc.subjectNMJen_US
dc.subjectDrosophilaen_US
dc.titleAn Investigation Of Vesicular Trafficking And Secretion In Type Iii Neuromuscular Junctions Of D. Melanogasteren_US
dc.typedissertation or thesisen_US
thesis.degree.disciplineGenetics
thesis.degree.grantorCornell Universityen_US
thesis.degree.levelDoctor of Philosophy
thesis.degree.namePh. D., Genetics
dc.contributor.chairDeitcher, David Lawrenceen_US
dc.contributor.committeeMemberRoberts, Jeffrey Warrenen_US
dc.contributor.committeeMemberBooker, Ronalden_US
dc.contributor.committeeMemberKnipple, Douglas Cen_US


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