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dc.contributor.authorHopkins, Jesse B.
dc.contributor.authorKatz, Andrea M.
dc.contributor.authorMeisburger, Steve P.
dc.contributor.authorWarkentin, Matthew A.
dc.contributor.authorThorne, Robert E.
dc.contributor.authorPollack, Lois
dc.date.accessioned2015-03-19T12:46:49Z
dc.date.available2015-03-19T12:46:49Z
dc.date.issued2015-02
dc.identifier.citationHopkins, J. B., Katz, A. M., Meisburger, S. P., Warkentin, M. A., Thorne, R. E. & Pollack, L. (2015) J. Appl. Cryst. 48, 227-237. doi:10.1107/S1600576714027782en_US
dc.identifier.citationHopkins, J. B., Katz, A. M., Meisburger, S. P., Warkentin, M. A., Thorne, R. E. & Pollack, L. (2015) J. Appl. Cryst. 48, 227-237. doi:10.1107/S1600576714027782/aj5246sup1.pdf
dc.identifier.urihttps://hdl.handle.net/1813/39215
dc.description.abstractThe application of small-angle X-ray scattering (SAXS) for high-throughput characterization of biological macromolecules in solution is limited by radiation damage. By cryocooling samples, radiation damage and required sample volumes can be reduced by orders of magnitude. However, the challenges of reproducibly creating the identically sized vitrified samples necessary for conventional background subtraction limit the widespread adoption of this method. Fixed path length silicon sample holders for cryoSAXS have been micro fabricated to address these challenges. They have low background scattering and X-ray absorption, require only 640 nl of sample, and allow reproducible sample cooling. Data collected in the sample holders from a nominal illuminated sample volume of 2.5 nl are reproducible down to q ’ 0.02 Å-1, agree with previous cryoSAXS work and are of sufficient quality for reconstructions that match measured crystal structures. These sample holders thus allow faster, more routine cryoSAXS data collection. Additional development is required to reduce sample fracturing and improve data quality at low q.en_US
dc.language.isoen_USen_US
dc.publisherJournal of Applied Crystallographyen_US
dc.subjectsaxsen_US
dc.subjectcryoSAXSen_US
dc.subjectsample holderen_US
dc.subject100 Ken_US
dc.subjectSASen_US
dc.subjectsmall angle x-ray scatteringen_US
dc.subjectbiological SAXSen_US
dc.subjectsolution scatteringen_US
dc.subjectfixed path lengthen_US
dc.titleData for "A microfabricated fixed path length silicon sample holder improves background subtraction for cryoSAXS"en_US
dc.typedataseten_US
dc.typearticle


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