Show simple item record

dc.contributor.authorHarden, Maegan
dc.date.accessioned2006-10-27T17:49:10Z
dc.date.available2006-10-27T17:49:10Z
dc.date.issued2006-11
dc.identifier.citationHarden MV, Newton LA, Lloyd RC, Whitlock KE. Olfactory imprinting is correlated with changes in gene expression in the olfactory epithelia of the zebrafish. J Neurobiol. 2006 Nov;66(13):1452-66.en_US
dc.identifier.otherbibid: 6476221
dc.identifier.urihttps://hdl.handle.net/1813/3697
dc.descriptionKathleen Whitlock, Ph.D.- advisor, Kenneth Kemphues, Ph.D.- committee member, David Lin, Ph.D- committee memberen_US
dc.description.abstractI have used the zebrafish, Danio rerio to study the following stages of olfactory sensory system development: the cell movements underlying olfactory placode (OP) formation, environmentally induced gene expression changes in the differentiating OP and modulation of gene expression changes in sensory neuron development. Cranial neural crest (CNC) and placodes both contribute to vertebrate sensory structures. Little is understood about the extent of cellular mixing between the CNC and OP fields during OP formation. I used live imaging and molecular markers in fixed tissue to follow the CNC and OP fields during OP formation. I found that while the CNC cells associate with and eventually surround the OP, little cell mixing occurs between the fields during this process. The OP develops into the olfactory epithelium (OE). I explored the effects of the nenvironment on gene expression in the developing OE. My lab previously showed that zebrafish form and retain olfactory memories of the odorant phenylethyl alcohol (PEA). I performed microarray analysis using the OEs of PEA imprinted fish to identify upregulated genes. One of the genes I identified was the transcription factor otx2. Otx2 is expressed in the developing and adult OE. The number of otx2 expressing cells is significantly increased in juvenile and adult OE of PEA exposed fish. I showed that otx2 cells also express neuronal markers suggesting that PEA exposure leads to an expansion of a neuronal precursor population that is maintained throughout life. The environmentally induced gene expression changes I observed could be a consequence of activating the immediate early genes (IEGs), which are transcription factors that are rapidly upregulated in response to sensory stimuli. To test if IEG expression was affected by odorant exposure my lab and I exposed juvenile zebrafish to a number of odorants and examined the expression of three IEGs in the developing OE. We found a significant change in the expression of the IEG, c-fos when fish were exposed to odorants of behavioral relevance. My studies provide a better understanding of how the OP forms and how the environment affects the differentiation of cells within the OE.en_US
dc.description.sponsorshipField of Genetics and Development NIH Training Grant (GM07617), GAANN (Graduate Assistants in Areas of National Need) Fellowship P200A000118, Cornell Center for Vertebrate Genomics Graduate Research Fellowship, NIH Grant R01 DC04218 (awarded K. Whitlock)en_US
dc.format.extent28492 bytes
dc.format.extent44965 bytes
dc.format.extent1743354 bytes
dc.format.extent34141 bytes
dc.format.extent1859164 bytes
dc.format.extent48964 bytes
dc.format.extent18837354 bytes
dc.format.extent51559 bytes
dc.format.extent33214091 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherJohn Wiley & Sons, Incen_US
dc.subjectZebrafish, Danio rerioen_US
dc.subjectDevelopmental biologyen_US
dc.subjectolfactory systemen_US
dc.subjectmolecular biologyen_US
dc.titleIntrinsic and Extrinsic Contributions to the Development of the Zebrafish Olfactory Epitheliumen_US
dc.typedissertation or thesisen_US


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Statistics