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dc.contributor.authorLee, Jinwooken_US
dc.date.accessioned2013-07-23T18:23:40Z
dc.date.available2016-06-01T06:15:40Z
dc.date.issued2011-01-31en_US
dc.identifier.otherbibid: 8213839
dc.identifier.urihttps://hdl.handle.net/1813/33544
dc.description.abstract'Empire' apples are susceptible to firm flesh browning when stored at temperatures close to 0 oC, or after 1-methylcyclopropene (1-MCP) treatment when stored at warmer temperatures such as 3 or 4 oC. This study was designed to examine the effects of 1-MCP on metabolic responses of fruit stored at 2 kPa O2/2 kPa CO2 at either 0.5 or 3.3 oC for up to 40 weeks. The incidence of flesh browning was higher in 1-MCP treated fruit than in untreated fruit at either storage temperature. 1-MCP reduced nitroblue tetrazolium reducing activity but inconsistently affected H2O2 concentrations. 1-MCP increased malondialdehyde concentrations at 0.5 oC but not at 3.3 oC. 1-MCP treated fruit had lower ascorbic acid concentrations at the end of storage. 1-MCP resulted in lower glutathione concentrations but higher oxidized glutathione concentrations at the end of storage at 3.3 oC. While 1-MCP reduced copper/zinc-superoxide dismutase activity at 3.3 oC, it increased peroxidase activity at 3.3 oC. Overall, however, 1-MCP did not affect the activities of the other antioxidant enzymes consistently, and no direct association between antioxidant metabolism and flesh browning development was revealed. Partial least squares discriminant analysis revealed different metabolic divergence and separation by 1-MCP and storage temperature. Regardless of 1-MCP and storage temperature, malic, succinic and 2oxoglutaric acid concentrations decreased, while carbohydrates were not affected. 1MCP increased sorbitol, tryptophan, phenylalanine, glutamate, 5-oxoproline, aspartate, homoserine, threonine, isoleucine, valine, leucine, serine, chlorogenic acid, phloridzin and catechin concentrations at 3.3 oC. 1-MCP reduced volatile compounds, and levels were highest in untreated fruit at 3.3 oC. The partial least squares discriminant analysis loading plots indicated that sorbitol and [gamma]-aminobutyric acid could be associated with the development of flesh browning in 1-MCP treated fruit stored at 3.3 oC. Overall, the development of flesh browning is likely to be complex, resulting from the interaction of several metabolic pathways.en_US
dc.language.isoen_USen_US
dc.subject1-mcpen_US
dc.subjectflesh browningen_US
dc.subjectEmpireen_US
dc.subjectrosen_US
dc.subjectantioxidant systemen_US
dc.subjectmetabolomicsen_US
dc.subjectpls-daen_US
dc.titleMetabolic Alteration In 1-Mcp Treated 'Empire' Apples During Storageen_US
dc.typedissertation or thesisen_US
thesis.degree.disciplineHorticultural Biology
thesis.degree.grantorCornell Universityen_US
thesis.degree.levelDoctor of Philosophy
thesis.degree.namePh. D., Horticultural Biology
dc.contributor.chairWatkins, Christopher Brianen_US
dc.contributor.committeeMemberGibson, Donna Marieen_US
dc.contributor.committeeMemberDavies, Peter Johnen_US
dc.contributor.committeeMemberCheng, Lailiangen_US


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