Anti-flagellin antibodies inhibit motility in Roseburia intestinalis and Clostridium ramosum

Abstract

Here we show that antibodies with specificity to the flagellin protein of Roseburia hominis (Fla2) and Escherichia coli (FliC) are capable of inhibiting motility of cultured Roseburia intestinalis and Clostridium ramosum within 30 minutes of application. Anti-mouse antibodies are included as a specificity control.

Video 1. Clostridiumramosumanti-mouse30min. This is Clostridium ramosum 30 minutes after the addition of anti-mouse antibody. Motility is not noticeably altered.

Video 2. Clostridiumramosumcontrol30min. This is Clostridium ramosum without the addition of antibody. Motility is not noticeably altered.

Video 3. ClostridiumramosumFla230min. This is Clostridium ramosum 30 minutes after the addition of anti-Fla2 antibody. Motility is noticeably inhibited.

Video 4. ClostridiumramosumFliC30min. This is Clostridium ramosum 30 minutes after the addition of anti-FliC antibody. Motility is noticeably inhibited.

Video 5. Roseburiaintestinalisanti-mouse30min. This is Roseburia intestinalis 30 minutes after the addition of anti-mouse antibody. Motility is not noticeably altered.

Video 6. Roseburiaintestinaliscontrol30min. This is Roseburia intestinalis without the addition of antibody. Motility is not noticeably altered.

Video 7. RoseburiaintestinalisFla230min. This is Roseburia intestinalis 30 minutes after the addition of anti-Fla2 antibody. Motility is noticeably inhibited.

Video 8. RoseburiaintestinalisFliC30min. This is Roseburia intestinalis 30 minutes after the addition of anti-FliC antibody. Motility is noticeably inhibited.