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dc.contributor.authorTran, Thuaen_US
dc.date.accessioned2013-02-22T14:16:23Z
dc.date.available2017-09-26T06:01:00Z
dc.date.issued2012-05-27en_US
dc.identifier.otherbibid: 8251410
dc.identifier.urihttps://hdl.handle.net/1813/31498
dc.description.abstractDNA nanotechnology was used to interface with proteins through DNAprotein hybrid molecules for protein detection and protein intracellular delivery. EZZ protein, which can bind to almost all IgG antibodies, was selected to be conjugated to DNA linker. Different methods of DNA-protein conjugation were used to develop universal adapters between IgG antibodies and DNA. Using DNA nanobarcodes, which is based on the color ratios of two colors rather than using different colors, a library of IgG-based nanobarcodes were generated through a simple mixing step. The IgG-based nanobarcodes were used as a platform to simultaneously detect target proteins in different systems, including microbead-based, membrane-based, and tissue-based. Moreover, for the first time, a new concept of using DNA transfection reagents was introduced to deliver proteins intracellularly with great efficiency.en_US
dc.language.isoen_USen_US
dc.subjectNucleic Acidsen_US
dc.subjectProteinen_US
dc.subjectMaterialsen_US
dc.titleNucleic Acid-Protein Hybrid Materialsen_US
dc.typedissertation or thesisen_US
thesis.degree.disciplineAgricultural and Biological Engineering
thesis.degree.grantorCornell Universityen_US
thesis.degree.levelDoctor of Philosophy
thesis.degree.namePh. D., Agricultural and Biological Engineering
dc.contributor.chairLuo, Danen_US
dc.contributor.committeeMemberLis, John Ten_US
dc.contributor.committeeMemberMarch, John Cen_US


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