Show simple item record

dc.contributor.authorPerez, Joseen_US
dc.date.accessioned2013-01-31T19:44:06Z
dc.date.available2017-12-20T07:00:30Z
dc.date.issued2012-08-20en_US
dc.identifier.otherbibid: 7959793
dc.identifier.urihttps://hdl.handle.net/1813/31063
dc.description.abstractShort-chain carboxylic acids generated by various mixed- or pure-culture fermentation processes have been considered valuable precursors for production of bioalcohols. While conversion of carboxylic acids into alcohols is routinely performed via catalytic hydrogenation or with strong chemical reducing agents, biological conversion routes are being explored. Reduction of nbutyric acid by pure cultures using glucose as the source of energy and electrons have been studied. However, the high cost of sugars has lead to the searching for cheaper sources. Syngas produced via biomass gasification, which is a blend of carbon monoxide, hydrogen and carbon dioxide, was studied as an economical source of energy and electrons with pure cultures of Clostridium ljungdahlii as a biocatalyst for the carboxylic acids reduction. Acetic acid, propionic acid, n-butyric acid, isobutyric acid, n-valeric acid, and n-caproic acid were successfully converted into their corresponding alcohols. Furthermore, biomass yields and fermentation stoichiometry from our experimental data enabled us to amend thermodynamic calculations with the goal to evaluate how much metabolic energy C. ljungdahlii can generate during fermentation of carbon monoxide. Our results show ATP yield of 0.42 ATP per carbon monoxide molecule consumed for fermentation of syngas, and 0.38 ATP per carbon monoxide molecule consumed when n-butyrate is added to the growth medium. The ratio of protons pumped across the cell membrane vs. electrons transferred from ferredoxin to NAD+ via the RNF complex is suggested to be 1.0. The results obtained in this work suggest that the production of bioalcohols, based on the reduction of carboxylic acids, may be an attractive alternative industrial process.en_US
dc.language.isoen_USen_US
dc.subjectSyngas fermentationen_US
dc.subjectCarboxylic acid conversionen_US
dc.subjectClostridium ljungdahlen_US
dc.titleReduction Of Carboxylic Acids To Alcohols Using Syngas And Clostridium Ljungdahlii As Biocatalysten_US
dc.typedissertation or thesisen_US
thesis.degree.disciplineAgricultural and Biological Engineering
thesis.degree.grantorCornell Universityen_US
thesis.degree.levelMaster of Science
thesis.degree.nameM.S., Agricultural and Biological Engineering
dc.contributor.chairAngenent, Largusen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Statistics