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dc.contributor.authorLowe, Adamen_US
dc.identifier.otherbibid: 7955400
dc.description.abstractTargeted detection of bio-molecules in vivo and in the environment is a key area of research. Surface enhanced Raman spectroscopy (SERS) is emerging as a noninvasive platform for detection of a large variety of biomolecules. Here, bionanotechnology was investigated to further enhance the capabilities of SERS as a biosensor platform. Specifically, a system was developed using oligonucleotide labeled nanoparticles for multiplex detection of small nucleotide polymorphisms in the K-Ras oncogene at a 10 pM detection limit. The binding kinetics and fluorescence quenching of CTAB coated gold nanorods were also investigated for use in detection of hydrophobic biomolecules. Fluorescence quenching of Nile Red and IR-786 was found to be independent of binding affinity, as determined by surface plasmon resonance. A novel method of functionalizing proteins to nanoparticles was developed for use in SERS and other nanoparticles based platforms. The system uses selenocysteine mediated cleavage of a protein splicing element known as an intein. The selenocysteine activated protein can then be functionalized to any selenocysteine reactive surface or functional group in a pH dependent manner. After development using a maltose binding protein model, fluorescein labeling of an intein tagged single chain antibody against the A33 cancer antigen was confirmed by florescence activated cell sorting.en_US
dc.titleDevelopment Of Bio-Nanotechnology Systems For Sers Based Detection Of Bio-Moleculesen_US
dc.typedissertation or thesisen_US Universityen_US of Philosophy D., Microbiology
dc.contributor.chairBatt, Carl Aen_US
dc.contributor.committeeMemberNicholson, Linda Ken_US
dc.contributor.committeeMemberDelisa, Matthewen_US

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