JavaScript is disabled for your browser. Some features of this site may not work without it.
Development Of Bio-Nanotechnology Systems For Sers Based Detection Of Bio-Molecules
dc.contributor.author | Lowe, Adam | en_US |
dc.date.accessioned | 2012-12-17T13:50:34Z | |
dc.date.available | 2016-12-30T06:46:59Z | |
dc.date.issued | 2011-08-31 | en_US |
dc.identifier.other | bibid: 7955400 | |
dc.identifier.uri | https://hdl.handle.net/1813/30603 | |
dc.description.abstract | Targeted detection of bio-molecules in vivo and in the environment is a key area of research. Surface enhanced Raman spectroscopy (SERS) is emerging as a noninvasive platform for detection of a large variety of biomolecules. Here, bionanotechnology was investigated to further enhance the capabilities of SERS as a biosensor platform. Specifically, a system was developed using oligonucleotide labeled nanoparticles for multiplex detection of small nucleotide polymorphisms in the K-Ras oncogene at a 10 pM detection limit. The binding kinetics and fluorescence quenching of CTAB coated gold nanorods were also investigated for use in detection of hydrophobic biomolecules. Fluorescence quenching of Nile Red and IR-786 was found to be independent of binding affinity, as determined by surface plasmon resonance. A novel method of functionalizing proteins to nanoparticles was developed for use in SERS and other nanoparticles based platforms. The system uses selenocysteine mediated cleavage of a protein splicing element known as an intein. The selenocysteine activated protein can then be functionalized to any selenocysteine reactive surface or functional group in a pH dependent manner. After development using a maltose binding protein model, fluorescein labeling of an intein tagged single chain antibody against the A33 cancer antigen was confirmed by florescence activated cell sorting. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | sers | en_US |
dc.subject | intein | en_US |
dc.subject | ldr | en_US |
dc.title | Development Of Bio-Nanotechnology Systems For Sers Based Detection Of Bio-Molecules | en_US |
dc.type | dissertation or thesis | en_US |
thesis.degree.discipline | Microbiology | |
thesis.degree.grantor | Cornell University | en_US |
thesis.degree.level | Doctor of Philosophy | |
thesis.degree.name | Ph. D., Microbiology | |
dc.contributor.chair | Batt, Carl A | en_US |
dc.contributor.committeeMember | Nicholson, Linda K | en_US |
dc.contributor.committeeMember | Delisa, Matthew | en_US |