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dc.contributor.authorKrishnamoorthy, Kalyanaramanen_US
dc.date.accessioned2010-08-05T16:21:18Z
dc.date.available2015-08-05T06:22:47Z
dc.date.issued2010-08-05T16:21:18Z
dc.identifier.otherbibid: 6980408
dc.identifier.urihttps://hdl.handle.net/1813/17151
dc.description.abstractProtein thiocarboxylates are members of sulfur transfer protein family and have been shown to be involved in a variety of important biosynthetic pathways like vitamin B1, molybdopterin, cysteine, thionucleosides among many others. Despite their importance, there has been lack of systematic efforts towards identifying new thiocarboxylate-forming proteins. In this work, we have taken efforts in developing two strategies to label them in bacterial cell-free extracts using fluorescent tags. In addition, bioinformatics search for new thiocarboxylate-forming proteins using a genomic database, theseed.uchicago.edu and a protein database, Pfam, yielded a new methionine biosynthetic pathway that involves a protein thiocarboxylate as the sulfur donor to make the precursor, homocysteine. This discovery further validated the need for developing methods to identify, in cell-free extracts, proteins carrying this important post-translational modification. The sulfur source for the protein thiocarboxylate involved in the methionine biosynthetic pathway has also been identified as sulfate or sulfite.en_US
dc.language.isoen_USen_US
dc.titleProtein Thiocarboxylates: Proteomics, Mechanistic Studies And Pathway Discoveryen_US
dc.typedissertation or thesisen_US


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