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dc.contributor.authorRorabaugh, Jesseen_US
dc.date.accessioned2009-08-19T16:41:59Z
dc.date.available2014-08-19T06:20:14Z
dc.date.issued2009-08-19T16:41:59Z
dc.identifier.otherbibid: 6681415
dc.identifier.urihttps://hdl.handle.net/1813/13560
dc.description.abstractTwo potential methods to excise genes in a single cell by inserting Cre Recombinase into its nucleus are described here. Optical transfection using a femtosecond laser was found to be effective at getting dyes into cells, but because of issues with spontaneous transfection and cell death, was inadequate as a method to introduce plasmids into cells. Direct delivery of His-NLS-TAT Cre with a micropipette was able to excise the DNA in a small number of cells without causing significant damage to them. It however was unable to reproducibly localize the location of the cells produced to less than a 600 micron diameter circle on the plate.en_US
dc.language.isoen_USen_US
dc.titleOpto-Poration And Direct Uptake Of His-Tat-Nls-Cre As Single Cell Transfection Techniques For Cancer Initiationen_US
dc.typedissertation or thesisen_US


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