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Opto-Poration And Direct Uptake Of His-Tat-Nls-Cre As Single Cell Transfection Techniques For Cancer Initiation
dc.contributor.author | Rorabaugh, Jesse | en_US |
dc.date.accessioned | 2009-08-19T16:41:59Z | |
dc.date.available | 2014-08-19T06:20:14Z | |
dc.date.issued | 2009-08-19T16:41:59Z | |
dc.identifier.other | bibid: 6681415 | |
dc.identifier.uri | https://hdl.handle.net/1813/13560 | |
dc.description.abstract | Two potential methods to excise genes in a single cell by inserting Cre Recombinase into its nucleus are described here. Optical transfection using a femtosecond laser was found to be effective at getting dyes into cells, but because of issues with spontaneous transfection and cell death, was inadequate as a method to introduce plasmids into cells. Direct delivery of His-NLS-TAT Cre with a micropipette was able to excise the DNA in a small number of cells without causing significant damage to them. It however was unable to reproducibly localize the location of the cells produced to less than a 600 micron diameter circle on the plate. | en_US |
dc.language.iso | en_US | en_US |
dc.title | Opto-Poration And Direct Uptake Of His-Tat-Nls-Cre As Single Cell Transfection Techniques For Cancer Initiation | en_US |
dc.type | dissertation or thesis | en_US |