Tissue Engineering Applications of Polyhydroxybutyrate
Polyhydroxybutyrate (PHB) is an aliphatic biopolyester synthesized as a carbon and energy source by certain microorganisms with the enzyme PHA synthase. Due to its biocompatibility, PHB has been widely evaluated for a wide variety of tissue engineering applications. A method to extract the active PHA synthase enzyme and pattern it on various solid surfaces was established. Upon application of the enzyme?s substrate, PHB polymer granules were formed via enzyme-linked surface initiated polymerization, providing an in situ organic coating on a variety of inorganic solid surfaces. In this work, the suitability of this enzyme-linked surface initiated polymerization (ESIP) of PHB was evaluated in two applications: as a platform for the propagation of embryonic stem cells, and as a noninvasive means for bone repair.
Polyhydroxybutyrate; Surface Initiated Polymerization
articledissertation or thesis