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dc.contributor.authorCreque, Jacklyn
dc.date.accessioned2021-09-09T17:37:55Z
dc.date.available2021-09-09T17:37:55Z
dc.date.issued2021-05
dc.identifier.otherCreque_cornell_0058O_11141
dc.identifier.otherhttp://dissertations.umi.com/cornell:11141
dc.identifier.urihttps://hdl.handle.net/1813/109650
dc.description81 pages
dc.description.abstractWe examined the distribution of the dynein-associated protein NudE in Drosophila larval brain neuroblasts and spermatocytes, and analyzed the phenotypic consequences of a nudE null mutation. NudE can associate with kinetochores, spindles and the nuclear envelope. In nudE mutant brain mitotic cells, centrosomes are often detached from the poles. Moreover, the centrosomes of mutant primary spermatocytes do not migrate from the cell cortex to the nuclear envelope, establishing a new role for NudE. In mutant neuroblasts, chromosomes fail to congress to a tight metaphase plate, and cell division arrests because of spindle assembly checkpoint (SAC) activation. The targeting of NudE to mitotic kinetochores requires the dynein-interacting protein Lis1, and surprisingly Cenp-meta, a Drosophila CENP-E homolog. NudE is non-essential for the targeting of all mitotic kinetochore components tested. However, in the absence of NudE, the 'shedding' of proteins off the kinetochore is abrogated and the SAC cannot be turned off, implying that NudE regulates dynein function at the kinetochore.
dc.language.isoen
dc.titleROLES OF THE DROSOPHILA NUDE PROTEIN IN KINETOCHORE FUNCTION AND CENTROSOME MIGRATION
dc.typedissertation or thesis
thesis.degree.disciplineGenetics, Genomics and Development
thesis.degree.grantorCornell University
thesis.degree.levelMaster of Science
thesis.degree.nameM.S., Genetics, Genomics and Development
dc.contributor.chairGoldberg, Michael Lewis
dc.contributor.committeeMemberAlani, Eric
dcterms.licensehttps://hdl.handle.net/1813/59810
dc.identifier.doihttp://doi.org/10.7298/v4kv-9j93


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