To date there has not been rigorous analysis of promoter efficacy in Cannabis sativa L. Promoter-driven transgene expression has the potential to greatly speed development of C. sativa cultivars with novel traits. Methods to genetically engineer C. sativa and regenerate transgenic plants have been recently described in multiple publications, however these methods utilized standard promoters to drive transgenes, rather than native C. sativa promoters. In this study a patented C. sativa UBIQUITIN gene promoter (CsUBQprom) was cloned into two different plasmids containing different reporter genes for the purpose of characterizing the pattern of expression driven by CsUBQprom in C. sativa. Using conventional enzyme digest and ligation methods, the SiM24 promoter was replaced with CsUBQprom in pSiM24-GFP and pSiM24-GUS to construct pFAH101 and pFAH112, respectively. Research to develop a toolkit of native C. sativa promoters would facilitate further genetic engineering and genome editing studies and accelerate this approach to hemp breeding efforts in the future.