Eastern equine encephalitis virus (EEEV) is a mosquito-borne Alphavirus endemic to the eastern United States, Canada, and the Caribbean. EEEV infection results in serious neuroinvasive disease in dead-end hosts like horses and humans. Human cases are considered rare and occur irregularly in the Eastern United States, but numbers have increased in recent years. EEEV is maintained in an enzootic cycle between passerine bird reservoirs and the mosquito Culiseta melanura. Other species of mosquitoes, called bridge vectors, are responsible for EEEV infection in dead end hosts. Spatiotemporal variation in bridge vectors makes incrimination of any one species difficult during an outbreak. Currently, mosquito surveillance for EEEV involves testing pools of Cs. melanura for the virus. In a EEEV focus surrounding the Great Dismal Swamp in Southeast Virginia, the virus is regularly detected in pools of Cs. melanura, as well as in sentinel chickens. Despite local EEEV transmission, equine cases are rare, but regular, and human cases are extremely rare. My study evaluated collection putative bridge vectors, bloodmeal analysis to determine host feeding patters, arboviral testing, and analysis of ecological associations of Cs. melanura to determine the role of bridge vector species in southeast Virginia.A variety of mosquito collection methods including resting boxes, vegetation aspiration, barrier screens, and ramp traps were used to collect putative bridge vectors of EEEV. Vegetation aspiration was found to result in the highest overall numbers and diversity of putative bridge vector species. My study is the first to evaluate ramp traps and barrier screens for their use in EEEV surveillance; both are effective at collecting putative bridge vector species. Bloodmeal analysis revealed that the enzootic vector, Cs. melanura feeds on both passerine reservoirs of EEEV and sentinel chickens. Other putative bridge vector species were also found to feed on sentinel chickens and dead-end hosts. Spatial analysis of swamp habitat and collection of Cs. melanura revealed that Cs. melanura populations are likely fragmented in this area and that putative bridge vector species are not always associated with Cs. melanura. This study identifies a portion of the cycle of EEEV in an endemic area, allowing for the development of targeted and effective mosquito control strategies to prevent outbreaks of EEEV.