Much is known about the effects of matrix stiffness and matrix topography on cell behavior, however they are seldom investigated in tandem. Presented and validated here is a micromolding method to introduce topographical features onto polyacrylamide (PA) substrates of tunable Young's modulus (E ). Feature fidelity is largely influenced by hydrogel swelling and most efficient on gels with a crosslinker concentration above 4.5%. However, this swelling does not affect the spacing of surface patterned ligands on two-dimensional PA gels. Bovine aortic endothelial cells (BAECs) cultured on linear micromolded features exhibit greater degrees of contact guidance with greater substrate E . Also described are applications of micromolding for future studies in cell mechanics.