Understanding how adolescent tree species influence belowground carbon dynamics is essential for predicting forest responses to changing climate conditions. One such example includes root exudation (the process by which roots release carbon compounds into the surrounding environment). This plays a key role in mycorrhizal associations and tree survival, particularly during the vulnerable seedling stage (<6 months old). In this study, we measured total organic carbon (TOC) root exudation in six tree species native to the Northeastern United States. These species were selected for their varied mycorrhizal associations (arbuscular vs. ectomycorrhizal) and drought tolerance to assess the influence of functional traits and species mixing on belowground carbon allocation. Seedlings were assessed in monoculture and mixed-species assemblages under controlled greenhouse conditions. Our findings reveal species-specific differences in TOC exudation and suggest that interspecies interactions may influence carbon exudation rates, providing evidence for belowground synergistic effects. These results offer insights into future climate-resilient reforestation models.