During 2017 a second collection effort was performed to obtain data regarding aquatic macroinvertebrates and substrate characteristics at Hudson River tributary sites collected during initial study in 2016. During 2017, all five tributary sites were again sampled: Wynantskill, Annandale Dam – Sawkill; Shapp Pond – East Branch Wappingers Creek; Browns Pond – Otterkill; and Oscawana Dam – Furnace Brook (Figure 1). Since the 2016 sampling effort, the Shapp Pond dam was completely removed. Thus, 2017 sampling represents the first post-removal data at that site. The barrier on the Wynantskill was removed in May 2016, therefore the 2017 effort represents the second post-removal data collection at that site. Barriers at Brown’s Pond, Oscawana Dam, and Annandale Dam remained intact during the 2017 sampling effort. As such, 2017 collection efforts represent the second year of baseline pre-removal data at existing barrier sites.