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  4. Simulating the Limits of Multiphoton Microscopy

Simulating the Limits of Multiphoton Microscopy

File(s)
Rebec_cornell_0058O_11183.pdf (9.97 MB)
Permanent Link(s)
https://doi.org/10.7298/5wpj-zm98
https://hdl.handle.net/1813/109680
Collections
Cornell Theses and Dissertations
Author
Rebec, Mihailo Russell
Abstract

Multiphoton microscopy is an important tool for high-resolution imaging of turbid media. A fundamental limit of the technique is the excitation produced outside of the focal region. The ratio of the signal at the focus to the background--the signal-to-background ratio, or SBR--is one important quantity that determines the limit of depth that can be imaged in a multiphoton experiment. While it is often believed that only unscattered light contributes to this background, scattered light is also a significant component. In this thesis a convolutional method of solving for scattered light will be used and adjusted for the multiphoton problem. Additionally, a novel Monte Carlo formulation is implemented on the GPU for this same purpose. The two techniques give similar results for produced excitations and SBR. Both demonstrate the primary importance of scattered light in the generation of out-of-focus excitation. Each method also shows the strong dependence of the SBR on a variety of illumination and optical properties.

Description
100 pages
Date Issued
2021-05
Keywords
Microscopy
•
Monte Carlo
•
Multiphoton
Committee Chair
Xu, Chris
Committee Member
Schaffer, Chris
Degree Discipline
Applied Physics
Degree Name
M.S., Applied Physics
Degree Level
Master of Science
Type
dissertation or thesis
Link(s) to Catalog Record
https://newcatalog.library.cornell.edu/catalog/15049433

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