The main objective of this study is to explore the lipid metabolism of Y. lipolytica in response to inositol compared to S. cerevisiae under fermentation. Another goal is to study several key parameters such as specific growth rate and metabolite production of S. cerevisiae and Y. lipolytica under media condition with and without inositol. The study of these parameters is essential for a basic understanding of cellular responses in a lab bench fermenter and in application to scale up fermentation production if desired.