Ag Biologicals are widely used in vegetable crops. They are particularly advantageous when used in protected culture, i.e. within net houses, plastic houses, or glass houses, in which finer environmental control is possible, allowing the biologicals to be used more effectively. DNA-sequence-based information is available to identify pest targets and to provide active agents that knock out those targets. The pest may be a weed, insect or a pathogen—a virus or a fungus—and the ag biological shuts down a key pathway and controls the pest or pathogen.