This readme.txt file was generated on 2021-06-21 by Katherine Tschida The files included in this dataset include the data required to reproduce the analyses included in Zhao, Ziobro, Pranic et al., “Sex- and context-dependent effects of acute isolation on vocal and non-vocal social behaviors in mice.” PlosOne. Accepted. GENERAL INFORMATION 1. Title of Dataset: Data from: Sex- and context-dependent effects of acute isolation on vocal and non-vocal social behaviors in mice 2. Author Information A. Corresponding Author Contact Information Name: Katherine Tschida Institution: Cornell University OCRID ID: 0000-0002-8171-1722 Email: kat227@cornell.edu B. Alternate Author Contact Information Xin Zhao (xz746@cornell.edu) Patryk Ziobro (pz258@cornell.edu) Nicole Pranic (nmp76@cornell.edu) 3. Date of data collection (single date, range, approximate date): 2020-2021 4. Geographic location of data collection: Ithaca, NY, United States of America SHARING/ACCESS INFORMATION 1. Licenses/restrictions placed on the data: Data are available under a Creative Commons Universal Public Domain Dedication (CC0 1.0); the data are openly available for re-use, modification and distribution; proper attribution to the original data creators is appreciated. Please cite this dataset as: Zhao, Xin, Patryk Ziobro, Nicole M. Pranic, Samantha Chu, Samantha Rabinovich, William Chan, Jennifer Zhao, Caroline Kornbrek, Zichen He, and Katherine A. Tschida (2021). Data from: Sex- and context-dependent effects of acute isolation on vocal and non-vocal social behaviors in mice [dataset]. Cornell University Library eCommons Repository. https://doi.org/10.7298/z4x4-0160. 2. Links to publications that cite or use the data: Zhao, Xin, Patryk Ziobro, Nicole M. Pranic, Samantha Chu, Samantha Rabinovich, William Chan, Jennifer Zhao, Caroline Kornbrek, Zichen He, and Katherine A. Tschida (2021). Sex- and context-dependent effects of acute isolation on vocal and non-vocal social behaviors in mice. PlosOne. Accepted. DATA & FILE OVERVIEW 1. Data overview: This dataset has five types of data: (1) Audio files (.wav) recorded while pairs of mice engaged in social encounters, analyzed in Figures 1 and 3 and supplemental figures S1-S7 of Zhao, Ziobro, Pranic et al.; (2) Video files (.mp4) of those same social interactions, analyzed in Figure 2 and Figures S2-S7 of Zhao, Ziobro, Pranic et al.; (3) Matlab files (.mat) containing the times of manually scored ultrasonic vocalizations produced during social encounters (USV onsets), analyzed in Figures 1 and 3 and supplemental figures S1-S7 of Zhao, Ziobro, Pranic et al.; (4) Excel files (.xlsx) containing the detailed behavioral scoring of the social encounters, analyzed in Figure 2 and Figures S2-S7 of Zhao, Ziobro, Pranic et al.; (5) Excel files (.xlsx) containing the summary data used to create Figures 1-3 and Figure S1 of Zhao, Ziobro, Pranic et al. See additional information regarding File Organization at the end of this readme document. METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: See preprint at doi: https://doi.org/10.1101/2021.04.02.438237 for methodological description. 2. Methods for processing the data: See preprint at doi: https://doi.org/10.1101/2021.04.02.438237 for data processing description. 3. Instrument- or software-specific information needed to interpret the data: Audio files: Requires software that can open/access .wav files. Because ultrasonic vocalizations are not audible to humans, we recommend using Raven (Cornell Lab of Ornithology), Matlab (Mathworks), Avisoft-SASLab Pro (Avisoft), or similar to visualize and analyze our .wav files. Video files: Requires software that can open .mp4 files (such as Windows Media Player (Microsoft) or Quicktime (Apple)) Matlab files: Matlab (Mathworks) DATA-SPECIFIC INFORMATION: Audio, video, and Matlab USV onset files are named as follows: Date_SocialContext_Condition_sex_mouseID SocialContext abbreviations are as follows: MM is male-male interaction, MF is male-female interaction, and FF is female-female interaction. Conditions are as follows: single refers to pairs of mice with a single-housed resident; group refers to pairs of mice with a group-housed resident. Sex refers to the sex of the resident animal. MouseID is the identifier information for each animal in our study. Due to the nature of the recordings, the audio (.wav; mouse vocalization, no spoken language) and video (files (.mp4, non-vocal social interactions of mice) in this dataset do not contain transcripts. In the Matlab USV onset files, the unit of measurement for manually scored USV times is seconds. Excel files containing the detailed behavioral scoring from all social encounters are named according to context and condition (male-male single, male-male group, male-female single, male-female group, female-female single, and female-female group). Within each excel file, each tab contains the behavior scoring for a given social encounter in that context and condition. Please note that multiple trained observers performed the behavior scoring, and as such, there may be small differences from tab to tab in the precise columnar organization of the data. The unit of measurement for behavior scoring is seconds. Numerical codes used to score behavior are as follows: 0: not interacting 1: one or both mice circling around the top perimeter of the cage (these times are also included in the final time spent not interacting) 2: social investigation initiated by the resident 3: social investigation initiated by the visitor 4: mutual social investigation 5: mounting initiated by the resident 5.5: mounting initiated by the visitor 6: fighting initiated by the resident 6.5: fighting initiated by the visitor FILE ORGANIZATION Files in the eCommons Repository: ZhaoZiobroPranicEtAl_PlosOne2021_Readme.txt ZhaoZiobroPranicEtAl_PlosOne2021_MatlabAndExcelFiles.zip – contains all .mat, .xlsx and an archival bundle of the Excel files as .csv files with images and formulas. 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