This Hestrin_NewPhyt_2021_readme.txt file was generated on 2021.05.20 by Rachel Hestrin ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset: Data from: Plants and mycorrhizal symbionts acquire substantial soil nitrogen from gaseous ammonia transport 2. Author Information Principal Investigator Contact Information Name: Rachel Hestrin Institution: Cornell University, Lawrence Livermore National Laboratory Address: Physical and Life Science Directorate, Livermore, CA 94550, USA Email: rh482@cornell.edu, hestrin1@llnl.gov Associate or Co-investigator Contact Information Name: Peter Weber Institution: Lawrence Livermore National Laboratory Address: Physical and Life Science Directorate, Livermore, CA 94550, USA Email: weber21@llnl.gov Associate or Co-investigator Contact Information Name: Jennifer Pett-Ridge Institution: Lawrence Livermore National Laboratory Address: Physical and Life Science Directorate, Livermore, CA 94550, USA Email: pettridge2@llnl.gov Associate or Co-investigator Contact Information Name: Johannes Lehmann Institution: Cornell University Address: 915 Bradfield Hall; Ithaca, NY, 14853 Email: CL273@cornell.edu 3. Date of data collection: 2013.08.05 Ð 2019.05.23 4. Geographic location of data collection: Ithaca, NY; Livermore, CA 5. Information about funding sources that supported the collection of the data: This work was supported in part by the Cornell Atkinson Center for Sustainability. RH acknowledges support from the NSF IGERT Program (DGE-0903371 and DGE-1069193), NSF-BREAD (grant number IOS-0965336), and the NSF GRFP (DGE-1144153). This research was also supported in part by the U.S. Department of Energy Office of Biological and Environmental Research, Genomic Science Program LLNL Bioenergy Scientific Focus Area SCW1039. Work at LLNL was conducted under the auspices of DOE Contract DE-AC52-07NA27344. Special thanks to Akio Enders for help with experimental design, Kelly Hanley for help with sample collection, Kim Sparks and Cornell Stable Isotope Facility staff for help with IRMS analysis, Christina Ramon for help with NanoSIMS sample preparation, and Maria Harrison for providing the plant and fungal germplasm. -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: These data are shared under a Creative Commons Attribution 4.0 International License (CC BY 4.0). https://creativecommons.org/licenses/by/4.0/. 2. Links to publications that cite or use the data: Hestrin, R., Weber, P.K., Pett-Ridge, J. and Lehmann, J. (2021), Plants and mycorrhizal symbionts acquire substantial soil nitrogen from gaseous ammonia transport. New Phytologist. Accepted Author Manuscript. https://doi.org/10.1111/nph.17527 3. Links to other publicly accessible locations of the data: N/A 4. Links/relationships to ancillary data sets: N/A 5. Was data derived from another source? No If yes, list source(s): 6. Recommended citation for the data: Hestrin, Rachel, Weber, Peter K., Pett-Ridge, Jennifer, and Lehmann, Johannes. (2021) Plants and mycorrhizal symbionts acquire substantial soil nitrogen from gaseous ammonia transport. New Phytologist. [Dataset] Cornell University Library eCommons Repository. https://doi.org/10.7298/q898-5m03 --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: Hestrin_NewPhyt_NH3_gas_plants_fungi Short description: Includes measurements of biomass and nutrient content (N, C, 15N, 13C) of plant and fungal tissue harvested from a mesocosm experiment investigating plant and fungal uptake of N originating from NH3 gas B. Filename: Hestrin_NewPhyt_NH3_gas_plant_N_calculations Short description: Includes measurements of biomass and N content of plant tissue harvested from a mesocosm experiment investigating plant and fungal uptake of N originating from NH3 gas, including calculated proportional N uptake from NH3 C. Filename: Hestrin_NewPhyt_NH3_OM_plants Short description: Includes measurements of biomass and N content of plant harvested from a mesocosm experiment investigating plant uptake of N originating organic matter, including calculated proportional N uptake from organic matter D. Filename: Hestrin_NewPhyt_Soil_incubation Short description: Includes characteristics of soils incubated and measurements of CO2 and NH3 efflux from soils during a 14-day incubation ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Hestrin_NewPhyt_NH3_gas_plants_fungi Figures 1, 2, S1-S6 ----------------------------------------- 1. Number of variables: 11 2. Number of cases/rows: 90 3. Variable List A. Name: replicate Description: Description: Indicates which of the 6 replicated blocks the mesocosm was assigned to B. Name: AM Description: Indicates whether plants were grown with or without AMF -- yes -- no C. Name: pH Description: Indicates the pH of the middle mesocosm compartment separating the plants and fungi from the NH3 source -- 4 -- 7 -- 10 D. Name: type Description: Indicates the biomass type -- fungus: fungal hyphae extracted from soil -- root: plant roots -- shoot: plant shoots and other aboveground tissue E. Name: N_percent Description: Tissue N content (percent) F. Name: atom_percent_15N Description: Atom percent 15N of tissue sample G. Name: delta_15N Description: delta 15N of tissue sample in parts per mil H. Name: C_percent Description: Tissue C content (percent) I. Name: atom_percent_13C Description: Atom percent 13C of tissue sample J. Name: delta_13C Description: delta 13C of tissue sample in parts per mil K. Name: biomass_g Description: Biomass harvested from each tissue type -- na: no data available for fungal tissue ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Hestrin_NewPhyt_NH3_gas_plant_N_calculations Figures 1 & S1-S5, Table S1 ----------------------------------------- 1. Number of variables: 19 2. Number of cases/rows: 36 3. Variable List A. Name: replicate Description: Description: Indicates which of the 6 replicated blocks the mesocosm was assigned to B. Name: AM Description: Indicates whether plants were grown with or without AMF -- yes -- no C. Name: pH Description: Indicates the pH of the middle mesocosm compartment separating the plants and fungi from the NH3 source -- 4 -- 7 -- 10 D. Name: shoot_biomass_g Description: Biomass (in grams) of plant shoots and other aboveground tissues E. Name: shoot_N_percent Description: N content (percent) of plant shoots and other aboveground tissues F. Name: shoot_delta_15N Description: Delta 15N (in parts per mil) of plant shoots and other aboveground tissues G. Name: shoot_total_N_mg Description: Total N content (in mg) of plant shoots and other aboveground tissues H. Name: shoot_atom_percent_15N Description: Atom percent 15N of plant shoots and other aboveground tissues I. Name: shoot_15N_excess_mg Description: 15N excess (in mg) of plant shoots and other aboveground tissues; calculated by subtracting 0.369 from atom percent 15N to account for background levels of 15N in plant shoots and multiplying by shoot total N content J. Name: root_biomass_g Description: Biomass (in grams) of plant roots K. Name: root_N_percent Description: N content (percent) of plant roots L. Name: root_delta_15N Description: Delta 15N (in parts per mil) of plant roots M. Name: root_total_N_mg Description: Total N content (in mg) of plant roots N. Name: root_atom_percent_15N Description: Atom percent 15N of plant roots O. Name: root_15N_excess_mg Description: 15N excess (in mg) of root tissues; calculated by subtracting 0.368 from atom percent 15N to account for background levels of 15N in plant roots and multiplying by root total N content P. Name: plant_total_N_mg Description: Total N content (in mg) of above- and belowground plant tissues Q. Name: plant_total_15N_excess_mg Description: Total 15N excess (in mg) of above- and belowground plant tissues R. Name: plant_total_NH3_N_mg Description: Plant total N uptake from NH3; calculated by dividing total 15N excess by 0.98 to account for 98 atom % enrichment of NH3 gas injected into the mesocosms. S. Name: percent_NH3N_daily_plant_N Description: Percent of daily plant N acquired from NH3-N; calculated by dividing plant NH3 N uptake by total plant N uptake over a 3-day period. Total plant N uptake over a 3-day period was estimated by dividing total N uptake by 151 days (the length of the experiment) and multiplying by 3 days (the length of the NH3 exposure period). ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Hestrin_NewPhyt_NH3_OM_plants Figures 2, S1, S2, S5, & S6 ----------------------------------------- 1. Number of variables: 19 2. Number of cases/rows: 10 3. Variable List A. Name: replicate Description: Description: Indicates which of the 6 replicated blocks the mesocosm was assigned to B. Name: AM Description: Indicates whether plants were grown with or without AMF -- yes -- no C. Name: shoot_biomass_g Description: Biomass (in grams) of plant shoots and other aboveground tissues D. Name: shoot_N_percent Description: N content (percent) of plant shoots and other aboveground tissues E. Name: shoot_delta_15N Description: Delta 15N (in parts per mil) of plant shoots and other aboveground tissues F. Name: shoot_total_N_mg Description: Total N content (in mg) of plant shoots and other aboveground tissues G. Name: shoot_atom_percent_15N Description: Atom percent 15N of plant shoots and other aboveground tissues H. Name: shoot_15N_excess_mg Description: 15N excess (in mg) of plant shoots and other aboveground tissues; calculated by subtracting 0.3929 from atom percent 15N to account for background levels of 15N in plant shoots and multiplying by shoot total N content I. Name: root_biomass_g Description: Biomass (in grams) of plant roots J. Name: root_N_percent Description: N content (percent) of plant roots K. Name: root_delta_15N Description: Delta 15N (in parts per mil) of plant roots L. Name: root_total_N_mg Description: Total N content (in mg) of plant roots M. Name: root_atom_percent_15N Description: Atom percent 15N of plant roots N. Name: root_15N_excess_mg Description: 15N excess (in mg) of root tissues; calculated by subtracting 0.3917 from atom percent 15N to account for background levels of 15N in plant roots and multiplying by root total N content O. Name: plant_total_N_mg Description: Total N content (in mg) of above- and belowground plant tissues P. Name: plant_total_15N_excess_mg Description: Total 15N excess (in mg) of above- and belowground plant tissues Q. Name: percent_OM_N_taken_up Description: Proportion of organic matter N taken up by plants; calculated by divided total plant 15N excess by the quantity of 15N in the organic matter added to each mesocosm (0.1716689 mg 15N excess). R. Name: total_plant_N_from_OM_mg Description: Quantity of plant N acquired from organic matter; calculated by multiplying the percent of organic matter taken up (based on 15N) by the total quantity of organic matter N added to each mesocosm (34.46 mg N); assumes that the plants acquired 14N and 15N at the same ratio that was initially present in the organic matter. S. Name: percent_plant_N_from_OM Description: Percent of total plant N acquired from organic matter; calculated by dividing total plant N acquired from organic matter by total plant N. ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Hestrin_NewPhyt_Soil_incubation Figures 4 & S7, Table S2 ----------------------------------------- 1. Number of variables: 17 2. Number of cases/rows: 40 3. Variable List A. Name: replicate Description: Replicate (n = 4 per treatment) B. Name: location Description: Location from which soil sample was collected C. Name: climate Description: Climate at sampling site D. Name: biome Description: Biome at sampling site E. Name: clay Description: Percent clay content of soil F. Name: C_percent Description: Percent C content of soil G. Name: N_percent Description: Percent N content of soil H. Name: pH Description: pH of soil measured in 2N KCl at a ratio of 1:2 ?g ?mL?1 I. Name: soil_g_jar Description: Mass of soil in each incubation jar J. Name: NH3_ppm Description: NH3 ppm measured in 3 mL Boric acid trap inside jar after 14-day incubation K. Name: NH3_ug_g_soil Description: ug NH3 g-1 soil; calculated by converting ppm NH3 to ug NH3 captured in the acid trap and dividing by g soil per incubation jar L. Name: NH3_ug_g_soil_day Description: ug NH3 g-1 soil day-1; calculated by dividing ug NH3 g-1 soil by 14 days (the length of the incubation) M. Name: N_ng_kg_soil_day Description: ng N kg-1 soil day-1; calculated by scaling from ug g-1 to ng kg-1 and multiplying by the proportional contribution of N to total NH3 mass N. Name: CO2_mL Description: CO2 mL measured in KOH trap inside jar after 14-day incubation O. Name: CO2_mL_g_soil Description: CO2 mL g-1 soil; calculated by dividing total CO2 mL measured by g soil per incubation jar P. Name: CO2_mg_g_soil_day Description: CO2 mg g-1 soil day-1; calculated by converting mL CO2 to mg CO2 and dividing total CO2 mL g-1 soil by 14 days (the length of the incubation) Q. Name: C_mg_ton_soil_day Description: C mg ton-1 soil day-1; calculated by scaling from mg g-1 to mg ton-1 and multiplying by the proportional contribution of C to total CO2 mass